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KMID : 1037020220110020084
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Medical Lasers; Engineering, Basic Research, and Clinical Application
2022 Volume.11 No. 2 p.84 ~ p.91
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Photodynamic therapy for SNU-80 anaplastic thyroid cancer cells
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Woo Seung-Hoon
Kim Kun-Woo
Chung Phil-Sang
Lee Sang-Joon
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Abstract
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Background and Objectives: Anaplastic thyroid carcinoma (ATC) is one of the lethal human neoplasms. Photodynamic therapy (PDT) is based on the interaction of a photosensitizer and light with a specific wavelength, and induces destruction of cancer cells. The purpose of this study is to evaluate the effect of the PDT with photofrin on SNU-80 anaplastic thyroid cancer cells.
Materials and Methods: After PDT, cell viability was measured by MTT assay. The experiments were done at photofrin concentration of 1.6 to 3.2 μg/ml. Subcellular localization of photofrin were observed by ER-TrackerTM and MitoTracker®. Hoechst and propidium iodide (PI) double stained cells were analyzed to measure the cellular apoptosis and necrosis using confocal microscope at 4, 8, and 24 hours after laser irradiation. Caspase activation was measured by western blot analysis.
Results: Cytotoxicity was increased accordingly to increasing photofrin concentration in MTT assay. The photofrin was localized at cytoplasm and mitochondria inside SNU-80 cells. Apoptosis and necrosis cells were increased on confocal microscope, as time after irradiation was progressed. The expression of caspase-3, -9 and poly (ADP-ribose) polymerase (PARP) were detected by Western blot analysis indicating that Photoprin-PDT showed cytotoxicity to anaplastic thyroid cancer cells in dose-dependent pattern.
Conclusion: Photofrin-PDT led to in vitro cell death of SNU-80 cells through mitochondrial mediated apoptosis. PDT could be used as a part of multimodal treatment for ATC.
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KEYWORD
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Photodynamic therapy, Anaplastic thyroid carcinoma
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